Overview

  • Product nameAnti-EFS antibody
  • Description
    Mouse polyclonal to EFS
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-468 of Human EFS

  • Positive control
    • Transfected mammalian cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.

Target

  • RelevanceEFS is a docking protein which plays a pivotal role for cell adhesion-related intracellular tyrosine kinase signaling, where it may serve as an activator of SRC and is known to interat with the SH3 domain of FYN and with CRK, SRC, and YES.
  • Cellular localizationCytoplasm
  • Database links
  • Alternative names
    • Cas scaffolding protein family member 3 antibody
    • CAS3 antibody
    • CASS3 antibody
    • EFS1 antibody
    • EFS2 antibody
    • Embryonal Fyn associated substrate antibody
    • HEFS antibody
    • signal transduction protein (SH3 containing) antibody
    • SIN antibody
    • Src-interacting protein antibody
    see all

Anti-EFS antibody images

  • All lanes : Anti-EFS antibody (ab67502) at 1/500 dilution

    Lane 1 : EFS transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
    Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution


    Predicted band size : 59 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-EFS antibody (ab67502)

ab67502 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (293T)
Loading amount 25 µg
Specification 293T
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"