EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats.
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Immunohistochemistry analysis of formalin fixed and paraffin embedded Human brain tissue labeling EGF using ab171327 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-EGF antibody (ab171327)
Anti-EGF antibody (ab171327) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg