• Product nameAnti-EGF antibody
    See all EGF primary antibodies
  • Description
    Rabbit polyclonal to EGF
  • Tested applicationsSuitable for: WB, Neutralising, Indirect ELISA, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant Rat EGF.



Our Abpromise guarantee covers the use of ab77851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 6.2 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/ml).
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. In conjunction with compatible secondary reagents, ab77851 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21121876
IHC-P Use a concentration of 1 µg/ml.


  • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in diseaseHypomagnesemia 4
  • Sequence similaritiesContains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

References for Anti-EGF antibody (ab77851)

This product has been referenced in:
  • Li H  et al. Different expression patterns of growth factors in rat fetuses with spina bifida aperta after in utero mesenchymal stromal cell transplantation. Cytotherapy 16:319-30 (2014). IHC-P ; Rat . Read more (PubMed: 24364908) »
  • Williams CM  et al. Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays. Tissue Eng Part A 17:1055-68 (2011). ICC/IF ; Rat . Read more (PubMed: 21121876) »

See all 2 Publications for this product

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