• Product nameAnti-EGF antibody
    See all EGF primary antibodies
  • Description
    Rabbit polyclonal to EGF
  • Tested applicationsSuitable for: ELISA, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human EGF



Our Abpromise guarantee covers the use of ab94898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/0.5 - 1/2.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa. Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10.


  • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in diseaseHypomagnesemia 4
  • Sequence similaritiesContains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

References for Anti-EGF antibody (ab94898)

ab94898 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your answer.

We guarantee the ab9695 for WB under either reducing or non-reducing conditions. The free of charge replacement will also be covered by our Abcam guarantee so if you would experience problems we would be pleased to h...

Read More

Thank you for your email and the great effort to translate everything into English. I am very sorry, I confused the languages in your email and wrote you in German. I hope you will have it easier to read this email.

Thank you very much for con...

Read More

Vielen Dank für diese zusätzliche Information.

Können Sie mir bitte bestätigen, ob Sie für den sELISA auch den ab94898 verwenden? Das würde darauf hindeuten, dass dieser das EGF im denaturierten Zustand scheinbar ...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I would like to reassure you that this antibody is tested and covered by our guarantee for WB (dotBlot) and human...

Read More

Thank you for your enquiry.

I can confirm that this particular antibody is sourced externally. The information we have is the following:

'To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-...

Read More