Our Abpromise guarantee covers the use of ab94898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/0.5 - 1/2.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa. Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10.|
ab94898 has not yet been referenced specifically in any publications.