Overview

  • Product nameAnti-EGF antibody
    See all EGF primary antibodies
  • Description
    Goat polyclonal to EGF
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mEGF (mouse Epidermal Growth Factor)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

To detect mEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mEGF.

WB Use at an assay dependent concentration.

To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in diseaseHypomagnesemia 4
  • Sequence similaritiesContains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

References for Anti-EGF antibody (ab9862)

This product has been referenced in:
  • Pan Y  et al. The expression of epidermal growth factor (EGF) and its receptor (EGFR) during post-natal testes development in the yak. Reprod Domest Anim 49:970-6 (2014). IHC . Read more (PubMed: 25263304) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This product is a polyclonal or total IgG antibody. It should recognize all subtypes, although we do not perform epitope mapping here.  I'm sorry I couldn't be of more assistance. Please let me know if you have any qu...

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The molecular weight is aprox. 140-150kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"