Overview

  • Product nameAnti-EGF antibody (Biotin)
    See all EGF primary antibodies
  • Description
    Goat polyclonal to EGF (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mouse EGF

  • General notesab83520 is sterile filtered.

Properties

Applications

Our Abpromise guarantee covers the use of ab83520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse EGF.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse EGF.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 133 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
    • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
    • Involvement in diseaseHypomagnesemia 4
    • Sequence similaritiesContains 9 EGF-like domains.
      Contains 9 LDL-receptor class B repeats.
    • Post-translational
      modifications
      O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta urogastrone antibody
      • EGF antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    References for Anti-EGF antibody (Biotin) (ab83520)

    ab83520 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab83520.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"