Overview

  • Product name
  • Description
    Rabbit polyclonal to EGFL8
  • Specificity
    This antibody reacts with EGFL8.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: EELQPEQVAE LWGRGDRIES LSDQVLLLEE RLGACSCEDN SLGLGVNH, corresponding to C terminal amino acids 245-294 of Human EGFL8

  • Positive control
    • Jurkat cell lysate and human skeletal muscle tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    The function of EGFL8 remains unknown.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • C6orf8 antibody
    • EGF like domain containing protein 8 precursor antibody
    • EGF like domain multiple 8 antibody
    • Epidermal growth factor like protein 8 antibody
    • FLJ44493 antibody
    • MGC44938 antibody
    • MGC59719 antibody
    • Multiple EGF like domain protein 8 antibody
    • NG3 antibody
    • NG3 protein antibody
    • OTTHUMP00000062670 antibody
    • Vascular endothelial statin 2 antibody
    • VE statin 2 antibody
    see all

Images

  • Anti-EGFL8 antibody (ab58650) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling EGFL8 with ab58650 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References

ab58650 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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