Overview

  • Product name
    Anti-EGFP antibody [F56-6A1.2.3]
  • Description
    Mouse monoclonal [F56-6A1.2.3] to EGFP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Immunogen

    Full length protein corresponding to EGFP aa 1-239. enhanced green fluorescent protein (eGFP).
    Database link: CAD97424.1

  • Positive control
    • U2OS cells transfected with an EGFR-eGFP fusion protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    in vitro produced
  • Clonality
    Monoclonal
  • Clone number
    F56-6A1.2.3
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 27 kDa.
ICC/IF 1/20 - 1/100.

Target

  • Alternative names
    • eGFP antibody
    • Enhanced GFP antibody
    • Green Fluorescent Protein antibody

Images

  • Immunofluorescent analysis of U2OS cells transfected with an EGFR-EGFP fusion protein (formalin-fixed, 0.1% Triton X-100 permeabilized) using either natural fluorescence (green) or an anti-EGFP antibody (red). Cells were then labeled with ab184601 at 1/20 dilution followed with DyLight 550 goat anti-mouse IgG secondary antibody at 1/200 dilution. Nuclei (blue) were stained with Hoechst 33342 dye. 20X magnification.

  • All lanes : Anti-EGFP antibody [F56-6A1.2.3] (ab184601) at 1/1000 dilution

    Lane 1 : recombinant GFP at 1 µg
    Lane 2 : recombinant GFP at 0.5 µg
    Lane 3 : recombinant GFP at 0.25 µg
    Lane 4 : recombinant GFP at 0.125 µg
    Lane 5 : recombinant GFP at 0.0625 µg

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa

References

This product has been referenced in:
  • Huang CJ  et al. Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma. Int J Mol Med 39:1195-1205 (2017). IF ; Human . Read more (PubMed: 28339008) »
  • Daschil N & Humpel C Green-Fluorescent Protein(+) Astrocytes Attach to Beta-Amyloid Plaques in an Alzheimer Mouse Model and Are Sensitive for Clasmatodendrosis. Front Aging Neurosci 8:75 (2016). Read more (PubMed: 27092076) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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