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Full length protein corresponding to EGFP aa 1-239. enhanced green fluorescent protein (eGFP).
Database link: CAD97424.1
Our Abpromise guarantee covers the use of ab184601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 27 kDa.|
|ICC/IF||1/20 - 1/100.|
Immunofluorescent analysis of U2OS cells transfected with an EGFR-EGFP fusion protein (formalin-fixed, 0.1% Triton X-100 permeabilized) using either natural fluorescence (green) or an anti-EGFP antibody (red). Cells were then labeled with ab184601 at 1/20 dilution followed with DyLight 550 goat anti-mouse IgG secondary antibody at 1/200 dilution. Nuclei (blue) were stained with Hoechst 33342 dye. 20X magnification.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"