For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide within Human EGFR (phospho Y1068). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab40815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18948956|
|WB||1/500 - 1/5000. Predicted molecular weight: 170 kDa.Can be blocked with EGFR (phospho Y1068) peptide (ab190788).|
|ICC/IF||1/250 - 1/500.|
|IHC-P||1/250 - 1/500.|
Blocking/Diluting buffer 5% NFDM/TBST
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Dot blot analysis of EGFR (pY1068) peptide (Lane 1), SMAD5 (unmodified) peptide labelling EGFR (pY1068) with ab40815 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
ab40815 showing positive staining in Cervical carcinoma tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"