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Synthetic phospho-peptide corresponding to residues surrounding Tyr1092 of human EGFR.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
An immunizing peptide is available for this antibody as ab190788.
Our Abpromise guarantee covers the use of ab40815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18948956|
|WB||1/500 - 1/1000. Predicted molecular weight: 170 kDa.Can be blocked with EGFR (phospho Y1092) peptide (ab190788).|
|ICC/IF||1/250 - 1/500.|
|IHC-P||1/250 - 1/500.|
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Dot blot analysis of EGFR (pY1092) peptide (Lane 1), SMAD5 (unmodified) peptide labelling EGFR (pY1092) with ab40815 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
ab40815 staining chondrocytes in tissue section (E13.5) of mouse limb, by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval using 10mM Sodium Citrate buffer (pH 6.0) for 10 min at 95°C in a pressurized chamber and blocking in 5% serum for 1 hour at 25°C. Endogenous peroxidases were quenched with 3% H2o2 for 10 minutes. The primary antibody was diluted 1/250 in NGS buffer and incubated with sample for 8 hours at 4°C. A Biotin-conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary. 1XTBS/0.1% Tween-20 (TBST) gave minimum background over 1XPBS as wash buffer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"