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Synthetic phospho-peptide corresponding to residues surrounding Tyr1092 of human EGFR.
Produced under U.S. Patent No. 5,675,063.
An immunizing peptide is available for this antibody as ab190788.
Our Abpromise guarantee covers the use of ab40815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent dilution. PubMed: 18948956|
|WB||1/500 - 1/1000. Predicted molecular weight: 170 kDa.Can be blocked with EGFR (phospho Y1092) peptide (ab190788).|
|ICC/IF||1/250 - 1/500.|
|IHC-P||1/250 - 1/500.|
ab40815 staining chondrocytes in tissue section (E13.5) of mouse limb, by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval using 10mM Sodium Citrate buffer (pH 6.0) for 10 min at 95°C in a pressurized chamber and blocking in 5% serum for 1 hour at 25°C. Endogenous peroxidases were quenched with 3% H2o2 for 10 minutes. The primary antibody was diluted 1/250 in NGS buffer and incubated with sample for 8 hours at 4°C. A Biotin-conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary. 1XTBS/0.1% Tween-20 (TBST) gave minimum background over 1XPBS as wash buffer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"