Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
- Product nameAnti-EGFR (phospho Y1173) antibody [E124]See all EGFR primary antibodies ...
- DescriptionRabbit monoclonal [E124] to EGFR (phospho Y1173)
- SpecificityThe antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor)
- Tested applicationsWB, IHC-P, ICC, IP more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor.
- Positive control
- WB: EGF-treated A431 cells lysate IHC: Phosphatase-treated, paraffin-embedded skin carcinoma
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- Concentration information loading...
- Clonality Monoclonal
- Clone numberE124
Our Abpromise guarantee covers the use of ab32578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/25 - 1/50.
WB: 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
- Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
- Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
- Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
- Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.
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Anti-EGFR (phospho Y1173) antibody [E124] images
ab32578 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.
All lanes : Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution
Lane 1 : A431
Lane 2 : A431 + EGF cell lysates
Predicted band size : 170 kDa
Immunofluorescent staining of untreated A431 cells using ab32578.
Immunofluorescent staining of Phosphatase-treated A431 cells using ab32578.
References for Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
This product has been referenced in:
- Gerdes CA et al. GA201 (RG7160): a novel, humanized, glycoengineered anti-EGFR antibody with enhanced ADCC and superior in vivo efficacy compared with cetuximab. Clin Cancer Res 19:1126-38 (2013). Read more (PubMed: 23209031) »
- Hsieh TH et al. Phthalates stimulate the epithelial to mesenchymal transitionthrough an HDAC6-dependent mechanism in human breast epithelial stem cells. Toxicol Sci 128:365-76 (2012). Human . Read more (PubMed: 22552774) »