Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)

Overview

  • Product nameAnti-EGFR (phospho Y1173) antibody [E124]
    See all EGFR primary antibodies
  • Description
    Rabbit monoclonal [E124] to EGFR (phospho Y1173)
  • SpecificityThe antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor)
  • Tested applicationsWB, IHC-P, ICC, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor.

  • Positive control
    • WB: EGF-treated A431 cells lysate IHC: Phosphatase-treated, paraffin-embedded skin carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
IHC-P 1/25 - 1/50.
ICC 1/25 - 1/50.
IP 1/10.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho Y1173) antibody [E124] images

    • ab32578 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.
    • All lanes : Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution

      Lane 1 : A431
      Lane 2 : A431 + EGF cell lysates


      Predicted band size : 170 kDa
    • Immunofluorescent staining of untreated A431 cells using ab32578.
    • Immunofluorescent staining of Phosphatase-treated A431 cells using ab32578.

    References for Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)

    This product has been referenced in:
    • Chang YL  et al. Regulation of estrogen receptor a function in oral squamous cell carcinoma cells by FAK signaling. Endocr Relat Cancer 21:555-65 (2014). Human . Read more (PubMed: 24825747) »
    • Gerdes CA  et al. GA201 (RG7160): a novel, humanized, glycoengineered anti-EGFR antibody with enhanced ADCC and superior in vivo efficacy compared with cetuximab. Clin Cancer Res 19:1126-38 (2013). Read more (PubMed: 23209031) »

    See all 9 Publications for this product

    Product Wall

    This antibody is not a purified product. However the IgG concentration in the supernatant refers to the specific IgG.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"