The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
Use a concentration of 3 µg/ml.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Flow Cytometry-Egr1 antibody(ab54966)
Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Egr1 antibody (ab54966)
This product has been referenced in:
Gabriel KN et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol49:1541-1552 (2016).
Read more (PubMed: 27634112) »
Bakalash S et al. Egr1 expression is induced following glatiramer acetate immunotherapy in rodent models of glaucoma and Alzheimer's disease. Invest Ophthalmol Vis Sci52:9033-46 (2011).
Read more (PubMed: 21969301) »