Anti-EGR2 antibody [EPR4004] (ab108399)


  • Product nameAnti-EGR2 antibody [EPR4004]
    See all EGR2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4004] to EGR2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human EGR2.

  • Positive control
    • LnCaP, HepG2, MCF7, and SH SY5Y cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab108399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionSequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
    • Involvement in diseaseDefects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    • Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      Ubiquitinated by WWP2 leading to proteasomal degradation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AT591 antibody
      • CMT1D antibody
      • CMT4E antibody
      • DKFZp686J1957 antibody
      • E3 SUMO-protein ligase EGR2 antibody
      • Early growth response 2 antibody
      • Early growth response protein 2 antibody
      • EGR-2 antibody
      • egr2 antibody
      • EGR2_HUMAN antibody
      • FLJ14547 antibody
      • KROX 20 Drosophila homolog antibody
      • Krox 20 homolog Drosophila antibody
      • KROX-20, Drosophila, homolog (early growth response-2) antibody
      • KROX20 antibody
      • Krox20 protein antibody
      • Zinc finger protein Krox-20 antibody
      see all

    Anti-EGR2 antibody [EPR4004] images

    • Overlay histogram showing HepG2 cells stained with ab108399 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108399, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-EGR2 antibody [EPR4004] (ab108399) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : SH SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 53 kDa

    References for Anti-EGR2 antibody [EPR4004] (ab108399)

    ab108399 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - Triton X-100
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde

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    Verified customer

    Submitted Jul 06 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (brain)
    Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount 30 µg
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Jun 03 2016

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (U2OS cells)
    Specification U2OS cells
    Treatment 50 pM TGF-beta time course
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Sep 03 2014