Overview

  • Product nameAnti-EGR3 antibody
    See all EGR3 primary antibodies
  • Description
    Rabbit polyclonal to EGR3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human EGR3.

    (Peptide available as ab91443.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; THP1.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • RelevanceEGR3 is a member of the early growth response transcription factor family of C2H2 zinc finger proteins (other members EGR1, EGR2 and EGR4). EGR proteins are immediate early proteins, expression of which is swiftly upregulated in response to a wide range of extracellular stimuli. EGR3 is also thought to be involved in development of muscle spindles, and is upregulated in several regions of the brain in response to stress or injury.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Early growth response 3 antibody
    • Early growth response gene 3 antibody
    • Early growth response protein 3 antibody
    • Early growth responsive 3 antibody
    • EGR 3 antibody
    • MGC138484 antibody
    • Pilot antibody
    • Zinc finger protein pilot antibody
    see all

Anti-EGR3 antibody images

  • All lanes : Anti-EGR3 antibody (ab75461) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 12 minutes
  • ICC/IF image of ab75461 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75461, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.

References for Anti-EGR3 antibody (ab75461)

This product has been referenced in:
  • Kearney SJ  et al. Type I IFNs downregulate myeloid cell IFN-? receptor by inducing recruitment of an early growth response 3/NGFI-A binding protein 1 complex that silences ifngr1 transcription. J Immunol 191:3384-92 (2013). Read more (PubMed: 23935197) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"