Overview

  • Product nameAnti-EGR3 antibody
    See all EGR3 primary antibodies
  • Description
    Rabbit polyclonal to EGR3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (FSGSSDSVVH YNQMATENVM DIGLTNEKPN PELSYSGSFQ PAPGNKTVTY) of Human EGR3 (NP_004421).

  • Positive control
    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceEGR3 is a member of the early growth response transcription factor family of C2H2 zinc finger proteins (other members EGR1, EGR2 and EGR4). EGR proteins are immediate early proteins, expression of which is swiftly upregulated in response to a wide range of extracellular stimuli. EGR3 is also thought to be involved in development of muscle spindles, and is upregulated in several regions of the brain in response to stress or injury.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Early growth response 3 antibody
    • Early growth response gene 3 antibody
    • Early growth response protein 3 antibody
    • Early growth responsive 3 antibody
    • EGR 3 antibody
    • MGC138484 antibody
    • Pilot antibody
    • Zinc finger protein pilot antibody
    see all

Anti-EGR3 antibody images

  • Anti-EGR3 antibody (ab94569) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling EGR3 with ab94569 at 5µg/ml.

References for Anti-EGR3 antibody (ab94569)

ab94569 has not yet been referenced specifically in any publications.

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