Overview

  • Product nameAnti-EGR4 antibody
    See all EGR4 primary antibodies
  • Description
    Rabbit polyclonal to EGR4
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide ELISA titre: 1/312500.

WB Use a concentration of 0.0625 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceEGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AT133 antibody
    • Early growth response protein 4 antibody
    • Nerve growth factor induced clone C antibody
    • NGFI C antibody
    • NGFIC antibody
    • PAT133 antibody
    see all

Anti-EGR4 antibody images

  • Anti-EGR4 antibody (ab50636) at 0.0625 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 51 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

References for Anti-EGR4 antibody (ab50636)

ab50636 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"