Anti-EHBP1L1 antibody (ab122557)
Key features and details
- Rabbit polyclonal to EHBP1L1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHBP1L1 antibody -
Description
Rabbit polyclonal to EHBP1L1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: IEVGLLGVLG IETGAAEGAI LGTQEIASRD SGVPGLEADT TGIQVKEVGG SEVPEIATGT AETEILGTQE IASRSSGVPG LESEVAGAQE TEVGGSGISG, corresponding to amino acids of Human EHBP1L1.
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Positive control
- ICC: A-431 cells. IHC-P: Human skeletal muscle, prostate and lymph node tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 254102 Human
- SwissProt: Q8N3D4 Human
- Unigene: 502867 Human
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Alternative names
- DKFZp762C186 antibody
- EH domain-binding protein 1-like protein 1 antibody
- EH1L1_HUMAN antibody
see all
Images
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122557 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph node tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue labeling EHBP1L1 with ab122557 at 1/5000. No positivity observed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122557 has not yet been referenced specifically in any publications.