Anti-EHD1 antibody [EPR4954] (ab109311)


  • Product name
    Anti-EHD1 antibody [EPR4954]
    See all EHD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4954] to EHD1
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human EHD1.

  • Positive control
    • WB: HepG2, Human testis, SW480, SK-OV-3 and HeLa lysates IHC-P: Human kidney and tonsil tissues ICC/IF: HeLa cells FC: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-EHD1 antibody (Alexa Fluor® 647) [EPR4954] (ab201354)

    Anti-EHD1 antibody (Alexa Fluor® 488) [EPR4954] (ab201538)

     Anti-EHD1 antibody (HRP) [EPR4954] (ab201861)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.



Our Abpromise guarantee covers the use of ab109311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/100 - 1/250.


Anti-EHD1 antibody [EPR4954] images

  • All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : Human testis lysates
    Lane 3 : SW480 cell lysates
    Lane 4 : SK-OV-3 cell lysates
    Lane 5 : HeLa cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 61 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using ab109311 at a 1/100 dilution.
  • Immunofluorescent staining of EHD1 in HeLa cells, using ab109311 at a 1/100 dilution.
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling EHD1 with unpurified ab109311 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using ab109311 at a 1/100 dilution.

References for Anti-EHD1 antibody [EPR4954] (ab109311)

This product has been referenced in:
  • Meng Q  et al. Mammalian Eps15 homology domain 1 promotes metastasis in non-small cell lung cancer by inducing epithelial-mesenchymal transition. Oncotarget 8:22433-22442 (2017). IHC-P ; Human . Read more (PubMed: 27531895) »
  • Gao Y  et al. Expression of TGFß-1 and EHD1 correlated with survival of non-small cell lung cancer. Tumour Biol 35:9371-80 (2014). Read more (PubMed: 24946721) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam regarding our EHD1 antibody, ab109311. I have confirmed with the laboratory that this antibody is not predicted to cross react with other EHD proteins EHD2, 3, and 4. Sequence analysis shows that the immunogen shares n...

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