Overview

  • Product nameAnti-EHD1 antibody [EPR4955]
    See all EHD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4955] to EHD1
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 390-415 of Human EHD1

  • Positive control
    • HepG2, HeLa and JAR cell lysates; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P.
  • Target

    Anti-EHD1 antibody [EPR4955] images

    • Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 61 kDa
    • ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.

    References for Anti-EHD1 antibody [EPR4955] (ab109747)

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"