Overview

  • Product nameAnti-EHD2 antibody
    See all EHD2 primary antibodies
  • Description
    Mouse monoclonal to EHD2
  • Tested applicationsSuitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 365-428 of Human EHD2 with proprietary 26 kDa tag (NP_055416).

  • Positive control
    • EHD2 transfected HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionPlays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
  • Tissue specificityHighly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
  • Sequence similaritiesContains 1 EF-hand domain.
    Contains 1 EH domain.
  • DomainThe EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
  • Cellular localizationCell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • EH domain containing 2 antibody
    • EH domain containing 3 antibody
    • EH domain containing protein 2 antibody
    • EH domain-containing protein 2 antibody
    • EHD 2 antibody
    • Ehd2 antibody
    • EHD2_HUMAN antibody
    • EHD3 antibody
    • FLJ96617 antibody
    • PAST 2 antibody
    • PAST homolog 2 antibody
    • PAST2 antibody
    see all

Anti-EHD2 antibody images

  • Anti-EHD2 antibody (ab118356) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 61 kDa
  • Anti-EHD2 antibody (ab118356) at 5 µg/ml + EHD2 transfected HeLa cell lysate at 25 µg

    Predicted band size : 61 kDa
  • Detection limit for recombinant tagged EHD2 is 0.1 ng/ml as a capture antibody.

References for Anti-EHD2 antibody (ab118356)

ab118356 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"