Overview

  • Product nameAnti-EHD2 antibody
    See all EHD2 primary antibodies
  • Description
    Goat polyclonal to EHD2
  • SpecificityThis antibody is expected to recognise EHD1 protein as well as EHD2.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-RLVPPSKRRHKGSA

    , corresponding to C terminal amino acids 529 - 542 of Human EHD2.

  • Positive control
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing EHD2. IF/ICC: A549 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20008164
ELISA 1/64000. Peptide ELISA
WB Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).

Target

  • FunctionPlays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
  • Tissue specificityHighly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
  • Sequence similaritiesContains 1 EF-hand domain.
    Contains 1 EH domain.
  • DomainThe EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
  • Cellular localizationCell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • EH domain containing 2 antibody
    • EH domain containing 3 antibody
    • EH domain containing protein 2 antibody
    • EH domain-containing protein 2 antibody
    • EHD 2 antibody
    • Ehd2 antibody
    • EHD2_HUMAN antibody
    • EHD3 antibody
    • FLJ96617 antibody
    • PAST 2 antibody
    • PAST homolog 2 antibody
    • PAST2 antibody
    see all

Anti-EHD2 antibody images

  • ICC/IF image of ab23935 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA  / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-EHD2 antibody (ab23935) at 0.003 µg/ml + Human Heart lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Anti-EHD2 antibody (ab23935) at 0.1 µg/ml + Human Placenta lysate in RIPA buffer at 35 µg

    Predicted band size : 61 kDa

References for Anti-EHD2 antibody (ab23935)

This product has been referenced in:

See all 9 Publications for this product

Product Wall

We have not tested this antibody with hamster, only human and mouse. The immunogen used to raise this antibody is RLVPPSKRRHKGSA and is 100% homologous with Chinese Hamster EDH1 (note that this antibody should recognize both EDH1 and EDH2).  Therefore,...

Read More
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 300 µg
Specification HeLa
Immuno-precipitation step Protein A
Username

Debora Rapaport

Verified customer

Submitted Feb 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"