Anti-EHD4 antibody (ab153892)
Key features and details
- Rabbit polyclonal to EHD4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-EHD4 antibody
See all EHD4 primary antibodies -
Description
Rabbit polyclonal to EHD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 52-315 of Human EHD4 (Uniprot ID: Q9H223).
-
Positive control
- 293T, A431, HeLa and HepG2 whole cell lysates; Human BT483 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/1000. |
Target
-
Relevance
EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway. -
Cellular localization
Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. -
Database links
- Entrez Gene: 505206 Cow
- Entrez Gene: 30844 Human
- Entrez Gene: 98878 Mouse
- Entrez Gene: 192204 Rat
- Entrez Gene: 380399 Xenopus laevis
- Entrez Gene: 100125054 Xenopus tropicalis
- Omim: 605892 Human
- SwissProt: Q9H223 Human
see all -
Alternative names
- EH domain containing 4 antibody
- EH domain containing protein 4 antibody
- EH domain-containing protein 4 antibody
see all
Images
-
All lanes : Anti-EHD4 antibody (ab153892) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue labeling EHD4 with ab153892 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab153892 has been referenced in 2 publications.
- Feng J et al. Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20. J Virol 92:N/A (2018). PubMed: 29695422
- Dutta S et al. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology. Cancer Res 76:418-28 (2016). WB ; Human . PubMed: 26560516