Overview

  • Product name
  • Description
    Rabbit polyclonal to EHD4
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (LMNLISQEET STPTQLVQGG AFDGTTEGPF NQGYGEGAKE GADEEEWVVA) of Human EHD4 (NP_644670).

  • Positive control
    • Human fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway.
  • Cellular localization
    Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • EH domain containing 4 antibody
    • EH domain containing protein 4 antibody
    • EH domain-containing protein 4 antibody
    • EH domaincontaining protein 4 antibody
    • EH-domain containing 4 antibody
    • EHdomain containing 4 antibody
    • EHdomain containing4 antibody
    • Hepatocellular carcinoma associated protein 10/11 antibody
    • hepatocellular carcinoma-associated protein 10/11 antibody
    • hepatocellular carcinoma-associated protein HCA11 antibody
    • HGNC:3245 antibody
    • Ortholog of rat pincher antibody
    • PAST homolog 4 antibody
    • PAST4 antibody
    see all

Images

  • Anti-EHD4 antibody (ab83859) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83859 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83859, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab83859 at 5 µg/ml staining EHD4 in Human lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83859 at 5 µg/ml staining EHD4 in Human thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab83859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83859.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up