• Product name
  • Description
    Rabbit polyclonal to EHF
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: PFQEFDINGE HLCSMSLQEF TRAAGTAGQL LYSNLQHLKW NGQCSSDLFQ, corresponding to N terminal amino acids 74-123 of Human EHF



Our Abpromise guarantee covers the use of ab24337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • Function
    Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
  • Tissue specificity
    Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Domain
    The PNT domain acts as a transcriptional activator.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • EHF antibody
    • EHF_HUMAN antibody
    • Epithelium-specific Ets transcription factor 3 antibody
    • ESE-3 antibody
    • ESE3 antibody
    • ESE3 transcription factor antibody
    • ESE3B antibody
    • ESEJ antibody
    • Ets domain transcription factor antibody
    • ETS domain-containing transcription factor antibody
    • ETS homologous factor antibody
    • hEHF antibody
    see all


  • Anti-EHF antibody (ab24337) at 1.25 µg/ml + Jurkat cell lysate

    HRP conjugated anti-Rabbit IgG at 1/50000-1/100000

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands. ab24337 was used at a dilution of 1.25 ug/ml in 5% skim milk / PBS buffer, with a secondary HRP conjugated anti-Rabbit IgG. Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EHF with ab24337 at 8µg/ml. Renal tubule epithelial cells labelled are indicated with arrows. Magnification: X400.


ab24337 has not yet been referenced specifically in any publications.

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