Overview

  • Product nameAnti-EHHADH antibody [7F6AF11]
    See all EHHADH primary antibodies
  • Description
    Mouse monoclonal [7F6AF11] to EHHADH
  • Tested applicationsSuitable for: IP, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human liver mitochondria.

  • Positive control
    • Human liver mitochondria and HpG2 cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC Use a concentration of 1 µg/ml.

Target

  • Tissue specificityLiver and kidney. Lower amounts seen in the brain.
  • PathwayLipid metabolism; fatty acid beta-oxidation.
  • Sequence similaritiesIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • Post-translational
    modifications
    Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • Cellular localizationPeroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

Anti-EHHADH antibody [7F6AF11] images

  • EHHADH immunocaptured from Human liver mitochondria using ab110299.
  • Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.

References for Anti-EHHADH antibody [7F6AF11] (ab110299)

ab110299 has not yet been referenced specifically in any publications.

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