This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product nameAnti-EHM2 antibody
    See all EHM2 primary antibodies
  • Description
    Goat polyclonal to EHM2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • SpecificityExpected to recognise both reported isoforms (NP_060894.2; NP_061987.3).
  • Species reactivity

    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-HNYQPQYHPNIHPSQ

    , corresponding to internal sequence amino acids 445-459 of Human EHM2 (NP_060894.2; NP_061987.3).

  • Positive control
    • Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

  • Application notesPredicted molecular weight for endogenous protein: 100 kDa.
    Preliminary experiments gave an approx 48kDa band in Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates after 0.3µg/ml antibody staining. The 48kDa band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-EHM2 antibody (ab77484)

    This product has been referenced in:
    • Nakajima H & Tanoue T Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF. J Cell Biol 195:245-61 (2011). Read more (PubMed: 22006950) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"