Anti-EHMT1/GLP antibody (ab63161)

Overview

  • Product nameAnti-EHMT1/GLP antibody
    See all EHMT1/GLP primary antibodies
  • Description
    Rabbit polyclonal to EHMT1/GLP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human EHMT1/GLP aa 367-396 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9H9B1

  • Positive control
    • 293 cell lysates transiently transfected with the KMT1D / GLP / Eu HMTase1 gene.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab63161 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
ELISA 1/1000.

Target

  • FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • DomainThe ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA188C12.1 antibody
    • DKFZp667M072 antibody
    • EHMT 1 antibody
    • EHMT1 antibody
    • EHMT1_HUMAN antibody
    • Eu-HMTase1 antibody
    • Euchromatic histone lysine N methyltransferase 1 antibody
    • Euchromatic histone methyltransferase 1 antibody
    • Euchromatic histone-lysine N-methyltransferase 1 antibody
    • EUHMTASE1 antibody
    • FLJ12879 antibody
    • FP13812 antibody
    • G9a like protein 1 antibody
    • G9a like protein antibody
    • G9a-like protein 1 antibody
    • GLP 1 antibody
    • GLP antibody
    • GLP1 antibody
    • H3 K9 HMTase 5 antibody
    • H3-K9-HMTase 5 antibody
    • Histone H3 K9 methyltransferase 5 antibody
    • Histone H3-K9 methyltransferase 5 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
    • Histone-lysine N-methyltransferase EHMT1 antibody
    • KIAA1876 antibody
    • Lysine N methyltransferase 1D antibody
    • Lysine N-methyltransferase 1D antibody
    • RP11 188C12.1 antibody
    see all

Anti-EHMT1/GLP antibody images

  • All lanes : Anti-EHMT1/GLP antibody (ab63161) at 1/50 dilution

    Lane 1 : 293 cell lysates nontransfected
    Lane 2 : 293 cell lysates transiently transfected with the KMT1D / GLP / Eu HMTase1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 138 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EHMT1/GLP antibody (ab63161)

ab63161 has not yet been referenced specifically in any publications.

Product Wall

Thank you so much for your reply.
Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

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Thank you for your reply.
Yes, I think you are understanding this correctly.
I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

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