Anti-EHMT1/GLP antibody [B0422] - ChIP Grade (ab41969)


  • Product nameAnti-EHMT1/GLP antibody [B0422] - ChIP Grade
    See all EHMT1/GLP primary antibodies
  • Description
    Mouse monoclonal [B0422] to EHMT1/GLP - ChIP Grade
  • Tested applicationsSuitable for: ChIP, ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
    Database link: Q5DW34

  • Positive control
    • WT mouse ES cell



Our Abpromise guarantee covers the use of ab41969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 18066052
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa.
ELISA Use a concentration of 0.1 µg/ml.
IP Use at an assay dependent dilution.


  • FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • DomainThe ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA188C12.1 antibody
    • DKFZp667M072 antibody
    • EHMT 1 antibody
    • EHMT1 antibody
    • EHMT1_HUMAN antibody
    • Eu-HMTase1 antibody
    • Euchromatic histone lysine N methyltransferase 1 antibody
    • Euchromatic histone methyltransferase 1 antibody
    • Euchromatic histone-lysine N-methyltransferase 1 antibody
    • EUHMTASE1 antibody
    • FLJ12879 antibody
    • FP13812 antibody
    • G9a like protein 1 antibody
    • G9a like protein antibody
    • G9a-like protein 1 antibody
    • GLP 1 antibody
    • GLP antibody
    • GLP1 antibody
    • H3 K9 HMTase 5 antibody
    • H3-K9-HMTase 5 antibody
    • Histone H3 K9 methyltransferase 5 antibody
    • Histone H3-K9 methyltransferase 5 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
    • Histone-lysine N-methyltransferase EHMT1 antibody
    • KIAA1876 antibody
    • Lysine N methyltransferase 1D antibody
    • Lysine N-methyltransferase 1D antibody
    • RP11 188C12.1 antibody
    see all

Anti-EHMT1/GLP antibody [B0422] - ChIP Grade images

  • Ab41969 at 5 µg/ml staining WT mouse ES cell (Positive)
  • Ab41969 at 5µg/ml staining GLP KO mouse ES cell (Negative)

References for Anti-EHMT1/GLP antibody [B0422] - ChIP Grade (ab41969)

This product has been referenced in:
  • Ow JR  et al. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep 6:34163 (2016). IP . Read more (PubMed: 27667720) »
  • Balemans MC  et al. Reduced Euchromatin histone methyltransferase 1 causes developmental delay, hypotonia, and cranial abnormalities associated with increased bone gene expression in Kleefstra syndrome mice. Dev Biol 386:395-407 (2014). Mouse . Read more (PubMed: 24362066) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (B cells)
Negative control IgG
Specification B cells
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control Gene Promoter

Abcam user community

Verified customer

Submitted Apr 27 2017

Application Immunoprecipitation
Sample Human Cell lysate - nuclear (Dermal Endothelial Cells (Primary))
Total protein in input 300 µg
Immuno-precipitation step Protein A/G
Specification Dermal Endothelial Cells (Primary)

Abcam user community

Verified customer

Submitted Apr 24 2017

Application Western blot
Sample Human Cell lysate - nuclear (Dermal Endothelial cells (Primary))
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Specification Dermal Endothelial cells (Primary)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (C2C12 myoblast cells)
Specification C2C12 myoblast cells
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 07 2013

Thank you so much for your reply.
Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

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Thank you for your reply.
Yes, I think you are understanding this correctly.
I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

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Thank you for your reply and the additional information which will be very helpful to us to evaluate the antibody.
I have checked, but unfortunately we do not have another new lot for ab41969 in stock right now. Instead, I can send you a replaceme...

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Thank you for contacting us.
I am very sorry to hear that the antibody ab41969 is giving you problems.
Could you please let me know the following details for this antibody:
1) How were your samples prepared (e.g. lysis buffer)?
2) H...

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