Overview

  • Product nameAnti-EID1 antibody
    See all EID1 primary antibodies
  • Description
    Rabbit polyclonal to EID1
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    GSGSRELSLRPSRSGA

    conjugated to KLH, corresponding to C terminal amino acids 34-49 of Human EID1.

  • Positive control
    • EID1 transfected Cos1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 22 kDa.

Target

  • FunctionInteracts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
  • Tissue specificityWidely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
  • Developmental stageExpression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).
  • Post-translational
    modifications
    Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
  • Cellular localizationNucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 21 kDa pRb associated protein antibody
    • 21 kDa pRb-associated protein antibody
    • C15orf3 antibody
    • CBP antibody
    • Chromosome 15 open reading frame 3 antibody
    • CREBBP/EP300 inhibitor 1 antibody
    • CREBBP/EP300 inhibitory protein 1 antibody
    • CRI 1 antibody
    • CRI1 antibody
    • E1A like inhibitor of differentiation antibody
    • E1A-like inhibitor of differentiation 1 antibody
    • EID 1 antibody
    • EID-1 antibody
    • EID1 antibody
    • EID1_HUMAN antibody
    • EP300 interacting inhibitor of differentiation 1 antibody
    • EP300-interacting inhibitor of differentiation 1 antibody
    • IRO45620 antibody
    • MGC138883 antibody
    • MGC138884 antibody
    • NB4 apoptosis related protein antibody
    • PNAS 22 antibody
    • PNAS22 antibody
    • PTD014 antibody
    • Rb and p300 binding protein EID 1 antibody
    • Rb and p300 binding protein EID1 antibody
    • RBP21 antibody
    • Retinoblastoma protein associated protein antibody
    see all

References for Anti-EID1 antibody (ab14454)

ab14454 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire and for providing details of your Western blot protocol. I am very sorry to hear that you are having problem with this antibody. I have looked through your proc...

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Thank you for your enquiry regarding ab14454. We have not yet tested the cross-reactivity for this antibody with mouse tissue but expect the antibody to work as there is 90% similarity between mouse and human (which we have tested). Should you de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"