Recombinant
RabMAb

Anti-EID1 antibody [EPR13636] (ab180133)

Overview

  • Product name
    Anti-EID1 antibody [EPR13636]
    See all EID1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13636] to EID1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EID1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y6B2

  • Positive control
    • Human cervix carcinoma tissue; HeLa and HepG2 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab180133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 21 kDa.

Target

  • Function
    Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
  • Tissue specificity
    Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
  • Developmental stage
    Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).
  • Post-translational
    modifications
    Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
  • Cellular localization
    Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 21 kDa pRb associated protein antibody
    • 21 kDa pRb-associated protein antibody
    • C15orf3 antibody
    • CBP antibody
    • Chromosome 15 open reading frame 3 antibody
    • CREBBP/EP300 inhibitor 1 antibody
    • CREBBP/EP300 inhibitory protein 1 antibody
    • CRI 1 antibody
    • CRI1 antibody
    • E1A like inhibitor of differentiation antibody
    • E1A-like inhibitor of differentiation 1 antibody
    • EID 1 antibody
    • EID-1 antibody
    • EID1 antibody
    • EID1_HUMAN antibody
    • EP300 interacting inhibitor of differentiation 1 antibody
    • EP300-interacting inhibitor of differentiation 1 antibody
    • IRO45620 antibody
    • MGC138883 antibody
    • MGC138884 antibody
    • NB4 apoptosis related protein antibody
    • PNAS 22 antibody
    • PNAS22 antibody
    • PTD014 antibody
    • Rb and p300 binding protein EID 1 antibody
    • Rb and p300 binding protein EID1 antibody
    • RBP21 antibody
    • Retinoblastoma protein associated protein antibody
    see all

Images

  • All lanes : Anti-EID1 antibody [EPR13636] (ab180133) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 21 kDa
  • Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling EID1 using ab180133 at 1/100 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

References

ab180133 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180133.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up