Anti-EID3 antibody (ab124447)
Key features and details
- Rabbit polyclonal to EID3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EID3 antibody -
Description
Rabbit polyclonal to EID3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to amino acids 139-333 of Human EID3 (BC027612).
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Positive control
- Human fetal brain, liver, kidney lysates, HeLa, K562 and HepG2 cell lysates; fetasl testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Act as a repressor of nuclear receptor-dependent transcription possibly by interfering with CREBBP-dependent coactivation. May function as a coinhibitor of other CREBBP/EP300-dependent transcription factors. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Belongs to the NSE4 family. -
Cellular localization
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 493861 Human
- Entrez Gene: 66341 Mouse
- Entrez Gene: 691688 Rat
- Omim: 612986 Human
- SwissProt: Q8N140 Human
- SwissProt: Q3V124 Mouse
- SwissProt: Q4V8G2 Rat
- Unigene: 659857 Human
see all -
Alternative names
- E1A-like inhibitor of differentiation 3 antibody
- EID 3 antibody
- EID-1-like inhibitor of differentiation 3 antibody
see all
Images
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All lanes : Anti-EID3 antibody (ab124447) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HeLa cell lysate
Lane 5 : K562 cell lysate
Lane 6 : HepG2 cell lysate
Predicted band size: 38 kDa -
ab124447 at 1/100 dilution staining EID3 in Human fetal testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124447 has been referenced in 1 publication.
- Munakata K et al. Cancer Stem-like Properties in Colorectal Cancer Cells with Low Proteasome Activity. Clin Cancer Res 22:5277-5286 (2016). WB . PubMed: 27166395