Overview

  • Product name
    Anti-EIF1AY antibody
  • Description
    Rabbit polyclonal to EIF1AY
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-144 of Human EIF1AY (Uniprot ID: Q14602).

  • Positive control
    • Human Lung AdCA tissue; Raji and PC-12 whole cell lysate; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the eIF-1A family.
    Contains 1 S1-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 1A Y isoform antibody
    • eIF 4C antibody
    • eIF-1A Y isoform antibody
    • EIF-4C antibody
    • EIF1AY antibody
    • Eukaryotic translation initiation factor 1A antibody
    • Eukaryotic translation initiation factor 1A, Y chromosomal antibody
    • Eukaryotic translation initiation factor 1A, Y linked antibody
    • Eukaryotic translation initiation factor 4C antibody
    • IF1AY_HUMAN antibody
    • Y-chromosomal antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human Lung AdCA, labeling EIF1AY using ab155546 at 1/500 dilution.
  • Anti-EIF1AY antibody (ab155546) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 16 kDa
  • Anti-EIF1AY antibody (ab155546) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size : 16 kDa
  • Immunofluorescent analysis of methanol fixed A431 cells, labeling EIF1AY with ab155546 at 1/500 dilution (green). A431 cells were fixed in ice-cold Methanol for 5 min. Right panel shows Hoechst 33343 staining (blue).

References

ab155546 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab155546.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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