Overview

  • Product nameAnti-eIF2A antibody
    See all eIF2A primary antibodies
  • Description
    Rabbit polyclonal to eIF2A
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: DKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQ , corresponding to C terminal amino acids 505-554 of Human eIF2A.

  • Positive control
    • Fetal thymus cell lysate and skin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab55924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

Anti-eIF2A antibody images

  • Anti-eIF2A antibody (ab55924) at 2.5 µg/ml + Fetal thymus cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling eIF2A with ab55924 at 4-8µg/ml. Arrows indicate positively labelled epidermal cells. Magnification: 400X.

References for Anti-eIF2A antibody (ab55924)

ab55924 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam and thank you for bringing these issue to us. It does appear that these products should be listed as against eIF2A instead of eIF2 subunit alpha. I will make sure that this is brought to the attention of the appropria...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"