Validated using a knockout cell line
Recombinant
RabMAb

Anti-eIF2A antibody [EPR11041] (ab157478)

Overview

  • Product name
    Anti-eIF2A antibody [EPR11041]
    See all eIF2A primary antibodies
  • Description
    Rabbit monoclonal [EPR11041] to eIF2A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF2A. The exact sequence is proprietary.

  • Positive control
    • Ramos, MOLT4, U87-MG and Raji cell lysates; Raji cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab157478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
    • Tissue specificity
      Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
    • Sequence similarities
      Belongs to the WD repeat EIF2A family.
      Contains 3 WD repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • 65 kDa eukaryotic translation initiation factor 2A antibody
      • CDA 02 antibody
      • CDA02 antibody
      • EIF 2 antibody
      • EIF 2A antibody
      • eIF-2A antibody
      • EIF2 antibody
      • eif2a antibody
      • EIF2A_HUMAN antibody
      • Eukaryotic translation initiation factor 2A 65kDa antibody
      • Eukaryotic translation initiation factor 2A antibody
      • MST089 antibody
      • MSTP004 antibody
      • MSTP089 antibody
      see all

    Images



    • Predicted band size : 65 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
      Lane 3: U87-MG cell lysate (20 µg)
      Lane 4: MOLT4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab157478 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab157478 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab157478 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-eIF2A antibody [EPR11041] (ab157478) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 65 kDa
    • Immunofluorescent analysis of Raji cells labeling eIF2A with ab157478 at 1/100 dilution.

    References

    ab157478 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157478.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up