Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF2AK1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (TCSDEFSSLR LHHNRAITHL MRSAKERVRQ DPCEDISRIQ KIRSREVALE) of Human EIF2AK1 (NP_055228).

  • Positive control
    • Fetal lung lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
  • Tissue specificity
    Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 HRM (heme regulatory motif) repeats.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2AK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak1 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 1 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 1 antibody
    • HCR antibody
    • Heme controlled repressor antibody
    • Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody
    • Heme regulated inhibitor antibody
    • Heme regulated initiation factor 2 alpha kinase antibody
    • Heme regulated repressor antibody
    • Heme sensitive initiation factor 2a kinase antibody
    • Heme-controlled repressor antibody
    • Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody
    • Heme-regulated inhibitor antibody
    • Hemin sensitive initiation factor 2 alpha kinase antibody
    • Hemin-sensitive initiation factor 2-alpha kinase antibody
    • HRI antibody
    • KIAA1369 antibody
    • PRO1362 antibody
    see all

Images

  • ab84980 staining EIF2AK1 in lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84980 staining EIF2AK1 in brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Taniuchi S  et al. Integrated stress response of vertebrates is regulated by four eIF2a kinases. Sci Rep 6:32886 (2016). WB ; Mouse . Read more (PubMed: 27633668) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (HEK293T cell line)
Specification
HEK293T cell line
Blocking step
Blocking One as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

Submitted Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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