The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 5-15 µg/mg of lysate.
Is unsuitable for WB.
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Involvement in disease
Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
Belongs to the eIF-2B gamma/epsilon subunits family. Contains 1 W2 domain.
ab91563 at 10 µg/mg lysate precipitating eIF2b epsilon in HeLa whole cell lysate following IP. Lane 1; IP with an antibody which recognizes an upstream epitope of eIF2b epsilon. Lane 2; IP with ab91563. Lane 3; control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection by WB utilised the ECL with a 10 second exposure.