Anti-eIF2B epsilon (phospho S539) antibody (ab4775)


  • Product nameAnti-eIF2B epsilon (phospho S539) antibody
    See all eIF2B epsilon primary antibodies
  • Description
    Rabbit polyclonal to eIF2B epsilon (phospho S539)
  • SpecificityThis ab reacts with both human and rat (see, for example, PMID 21165642, 20484009 and 21832246) but it is not very clear if this recognition happens only when S535 (rat) and S544 (human) are phosphorylated or when S535 (rat)/S540 (human) are phosphorylated.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Rat) derived from the region of rat eIF-2B epsilon that contains serine 535 (corresponding to serine 539 in the human sequence).

  • Positive control
    • Recombinant eIF-2B epsilon treated with GSK 3 beta and lambda phosphatase, CHO-T cells transfected with human insulin receptor (IR) +/- insulin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eukaryotic initiation factor 2B epsilon (eIF-2B epsilon), and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using an eIF-2B epsilon-derived peptide that is phosphorylated at serine 539.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab4775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 82 kDa).


  • FunctionCatalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • Involvement in diseaseDefects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • Sequence similaritiesBelongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CACH antibody
    • CLE antibody
    • EI2BE_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor antibody
    • eIF 2B GDP GTP exchange factor subunit epsilon antibody
    • eIF-2B GDP-GTP exchange factor subunit epsilon antibody
    • EIF2B antibody
    • EIF2B5 antibody
    • EIF2BE antibody
    • EIF2Bepsilon antibody
    • Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody
    • LVWM antibody
    • Translation initiation factor eIF 2B subunit epsilon antibody
    • Translation initiation factor eIF-2B subunit epsilon antibody
    see all

Anti-eIF2B epsilon (phospho S539) antibody images

  • Predicted band size : 82 kDa

    Peptide Competition: Recombinant eIF-2B episilon treated with lambda phosphatase (1) or treated with GSK-3 beta (lanes 2-5) and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4775 antibody, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phophoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4775 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the up-regulation of the ab4775 phospho signal upon stimulation with GSK-3 beta.

References for Anti-eIF2B epsilon (phospho S539) antibody (ab4775)

This product has been referenced in:
  • Sidrauski C  et al. Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2:e00498 (2013). WB ; Human . Read more (PubMed: 23741617) »
  • Yung HW  et al. Evidence of placental translation inhibition and endoplasmic reticulum stress in the etiology of human intrauterine growth restriction. Am J Pathol 173:451-62 (2008). WB ; Human . Read more (PubMed: 18583310) »

See all 2 Publications for this product

Product Wall

Thank you for your interest and for passing the customer's details.
Expiration date: [31/05/2012]
I am very pleased to hear you would like to accept our offer and test ab4775 in Mouse. This code will give you 1 free ...

Read More

Thank you for your response.
I can certainly offer a discount code for testing and this case seems to be stronger since there may be some publication regarding the cross-reactivity with mouse (Western blot application).
Would you be so kind t...

Read More

Thank you for getting back to me. Thank you for confirming that both eIF2B and GSK3 are expressed in these cells. We recommend a positive control of recombinant eIF-2B epsilon treated with GSK 3 beta and lambda phosphatase or CHO-T cells transf...

Read More