Recombinant
RabMAb

Anti-eIF2B1 antibody [EPR13894(B)] (ab181186)

Overview

  • Product name
    Anti-eIF2B1 antibody [EPR13894(B)]
  • Description
    Rabbit monoclonal [EPR13894(B)] to eIF2B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF2B1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14232

  • Positive control
    • SH-SH5Y, Jurkat and MCF7 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13894(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/20 - 1/40.

Target

  • Relevance
    eIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
  • Cellular localization
    Plasma membrane
  • Database links
  • Alternative names
    • 26kDa antibody
    • D5Ertd406e antibody
    • EI2BA antibody
    • eIF 2a antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor subunit alpha antibody
    • EIF 2Balpha antibody
    • EIF2B alpha antibody
    • EIF2B antibody
    • EIF2B1 antibody
    • EIF2BA antibody
    • eukaryotic translation initiation factor 2B subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2B, alpha antibody
    • eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kD) antibody
    • Eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa antibody
    • MGC117409 antibody
    • MGC125868 antibody
    • MGC125869 antibody
    • MGC6458 antibody
    • Translation initiation factor eIF2B subunit alpha antibody
    • zgc:86660 antibody
    see all

Images

  • All lanes : Anti-eIF2B1 antibody [EPR13894(B)] (ab181186) at 1/10000 dilution

    Lane 1 : SH-SH5Y cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation. ab181186 at 1/1000 labeling elF2B1 immunoprecipitated from MCF7 cell lysate using ab181186 at 1/50.

References

ab181186 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181186.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up