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Chicken polyclonal to EIF2B2
Synthetic peptide, corresponding to N terminal amino acids 1-210 of Human EIF2B2.
E coli derived fusion protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 1 µg/ml.
WB: 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 40 kDa). We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Information by UniProt
EIF 2Bbeta antibody
Western blot - Anti-EIF2B2 antibody (ab37675)
Anti-EIF2B2 antibody (ab37675) at 1/2000 dilution + E coli derived fusion protein
Goat anti IgY HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size:
Observed band size:
80 kDa (
why is the actual band size different from the predicted?
The arrow next to the image points to the EIF2B2 fusion protien.
The use of a fusion protien may account for the difference observed in molecular weight compared to the native protien.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"