Anti-eIF2B3 antibody [1H3] (ab171093)
Key features and details
- Mouse monoclonal [1H3] to eIF2B3
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-eIF2B3 antibody [1H3] -
Description
Mouse monoclonal [1H3] to eIF2B3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human eIF2B3. Produced in HEK293T cells.
Database link: Q9NR50 -
Positive control
- HeLa, MCF7, K562, U2OS or NRK lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1H3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/200.
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WB |
1/1000. Predicted molecular weight: 50 kDa.
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IP |
Use at an assay dependent concentration.
3µg per 500µg lysate |
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ICC/IF |
1/10 - 1/100.
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Notes |
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IHC-P
1/100 - 1/200. |
WB
1/1000. Predicted molecular weight: 50 kDa. |
IP
Use at an assay dependent concentration. 3µg per 500µg lysate |
ICC/IF
1/10 - 1/100. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. -
Sequence similarities
Belongs to the eIF-2B gamma/epsilon subunits family. - Information by UniProt
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Database links
- Entrez Gene: 8891 Human
- Entrez Gene: 171145 Rat
- Omim: 606273 Human
- SwissProt: Q9NR50 Human
- SwissProt: P70541 Rat
- Unigene: 533549 Human
- Unigene: 10577 Rat
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Form
It localizes to the cytosol. -
Alternative names
- EI2BG_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit gamma antibody
see all
Images
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All lanes :
Lane 1 : MCF7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : U2OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : NIH3T3 whole cell lysate
Lane 9 : NRK whole cell lysate
Lysates/proteins at 80 µg per lane.
Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution
Predicted band size: 50 kDa -
Immunofluorescent analysis of eIF2B3 (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an eIF2B3 monoclonal antibody (ab171093) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunofluorescent analysis of eIF2B3 (green) showing staining in the cytoplasm of COS7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an eIF2B3 monoclonal antibody (ab171093) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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ab171093 staining eIF2B3 in the cytoplasm of Human uterus tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab171093 staining eIF2B3 in the cytoplasm of Human breast tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunoprecipitation of U2OS cells labeling eIF2B3 with ab171093 at 3µg per 500µg lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171093 has not yet been referenced specifically in any publications.