This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

  • Application notes

    This antibody gave a positive result in ELISA against the immunizing peptide (ab41550).
    Not yet tested in other applications.
  • Target

    • FunctionCatalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
    • Involvement in diseaseDefects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
    • Sequence similaritiesBelongs to the eIF-2B gamma/epsilon subunits family.
    • Information by UniProt
    • Database links
    • FormIt localizes to the cytosol.
    • Alternative names
      • EI2BG_HUMAN antibody
      • EIF 2B antibody
      • eIF 2B GDP GTP exchange factor subunit gamma antibody
      • eIF-2B GDP-GTP exchange factor subunit gamma antibody
      • eIF2B-gamma antibody
      • Eif2b3 antibody
      • EIF2Bgamma antibody
      • Eukaryotic translation initiation factor 2B subunit 3 gamma antibody
      • Eukaryotic translation initiation factor 2B, gamma antibody
      • eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa antibody
      • OTTHUMP00000010262 antibody
      • OTTHUMP00000010263 antibody
      • Translation initiation factor eIF-2B subunit gamma antibody
      see all

    Anti-eIF2B3 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-eIF2B3 antibody (ab41551) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
      Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
      Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human eIF2B3 peptide (ab41550) at 1 µg/ml
      Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human eIF2B3 peptide (ab41550) at 1 µg/ml
      Lane 8 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human eIF2B3 peptide (ab41550) at 1 µg/ml
      Lane 9 : HEK293 Human embryonic kidney cell line Whole Cell Lysate with Human eIF2B3 peptide (ab41550) at 1 µg/ml
      Lane 10 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate with Human eIF2B3 peptide (ab41550) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-eIF2B3 antibody (ab41551)

    ab41551 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"