Overview

  • Product name
  • Description
    Goat polyclonal to eIF2B4
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-RKDYGSKVSLFSH

    , corresponding to internal sequence amino acids 190-202 of Human eIF2B4 (NP_751945.2; NP_001029288.1; NP_056451.3).

  • Positive control
    • K562 lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).

Target

Images

  • Anti-eIF2B4 antibody (ab104941) at 0.3 µg/ml + K562 lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 hour


    An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.

References

ab104941 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104941.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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