Overview

  • Product nameAnti-EIF2G antibody
    See all EIF2G primary antibodies
  • Description
    Rabbit polyclonal to EIF2G
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse EIF2G.

    (Peptide available as ab33375.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MEF1, NIH3T3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • FunctioneIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. EIF2G subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-2-gamma X antibody
    • eIF-2gA antibody
    • eIF-2gX antibody
    • EIF2 antibody
    • EIF2G antibody
    • EIF2gamma antibody
    • EIF2S3 antibody
    • Eukaryotic translation initiation factor 2 gamma antibody
    • Eukaryotic translation initiation factor 2 gamma subunit antibody
    • Eukaryotic translation initiation factor 2 subunit 3 antibody
    • Eukaryotic translation initiation factor 2 subunit 3 gamma 52kDa antibody
    • Eukaryotic translation initiation factor 2 subunit gamma X antibody
    • IF2G_HUMAN antibody
    see all

Anti-EIF2G antibody images

  • All lanes : Anti-EIF2G antibody (ab33376) at 1/250 dilution

    Lane 1 : Mouse Embryonic Fibroblast 1
    Lane 2 : NIH 3T3 whole cell lysate (ab7179)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

References for Anti-EIF2G antibody (ab33376)

ab33376 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"