Overview

  • Product nameAnti-EIF2S1 antibody [EIF2S1A2B8]
    See all EIF2S1 primary antibodies
  • Description
    Mouse monoclonal [EIF2S1A2B8] to EIF2S1
  • Tested applicationsSuitable for: WB, Dot blot, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant Human EIF2S1

  • Positive control
    • HEK293 and NIH3T3 whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 36 kDa.
Dot blot Use at an assay dependent dilution.
ICC/IF 1/1.

Target

  • FunctionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similaritiesBelongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • Post-translational
    modifications
    Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • Cellular localizationCytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Anti-EIF2S1 antibody [EIF2S1A2B8] images

  • Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ab50733 at 1/1 dilution staining human EIF2S1 in HeLa Cells by Immunocytochemistry. A 1/200 dilution of Alexa Fluor 488 anti-mouse IgG is used as a secondary.
  • Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)

This product has been referenced in:
  • Yu Y  et al. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog 9:e1003266 (2013). WB ; Human . Read more (PubMed: 23592989) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"