Anti-EIF2S1 (phospho S51) antibody (ab47769)

Overview

  • Product nameAnti-EIF2S1 (phospho S51) antibody
    See all EIF2S1 primary antibodies
  • Description
    Rabbit polyclonal to EIF2S1 (phospho S51)
  • SpecificityThis antibody detects eIF2a only when phosphorylated at serine 51.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human EIF2S1 around the phosphorylation site of serine 51 (E-L-SP-R-R).

  • Positive control
    • K562 cell extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
ELISA 1/10000.

Target

  • FunctionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similaritiesBelongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • Post-translational
    modifications
    Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • Cellular localizationCytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Anti-EIF2S1 (phospho S51) antibody images

  • All lanes : Anti-EIF2S1 (phospho S51) antibody (ab47769) at 1/500 dilution

    Lane 1 : K562 cell extract, untreated.
    Lane 2 : K562 cell extract treated with IFN alpha (1000U/ml, 18 hours)


    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF2S1 (phospho S51) antibody (ab47769)

ab47769 has not yet been referenced specifically in any publications.

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