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Synthetic peptide (Human) derived from the region of human eIF-2 alpha that contains serine 51. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast.
Our Abpromise guarantee covers the use of ab4837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).|
|IHC-Fr||1/250. See Abreview.|
Extracts prepared from 3T3-L1 adipocytes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.
Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
1) the phosphopeptide immunogen
2) the non-phosphopeptide corresponding to the immunogen
3) no peptide
After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4837 blocks the antibody signal, thereby demonstrating the specificity of the antibody.