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Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

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Overview

Product name

Anti-EIF2S1 (phospho S51) antibody [E90]
See all EIF2S1 products (18) ...

Description

Rabbit monoclonal [E90] to EIF2S1 (phospho S51)

Specificity

The antibody only detects eIF-2a phosphorylated on Serine 51.

Tested applications

ICC/IF, WB, IHC-P, ICC, Dot Blot, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Fruit fly (Drosophila melanogaster), African Green Monkey, Neurospora crassa

Predicted to work with

Cow, Pig

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human EIF2S1.

Positive control

PC12 cell lysate, human liver carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

E90

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)Western blot - EIF2S1 (phospho S51) antibody [E90] (ab32157) image (enlarge)

  • - EIF2S1 (phospho S51) antibody [E90] (ab32157)- EIF2S1 (phospho S51) antibody [E90] (ab32157) image (enlarge)

  • Dot Blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)Dot Blot - EIF2S1 (phospho S51) antibody [E90] (ab32157) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Dot: 1/500.

ICC: 1/100 - 1/250.
ICC/IF: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent dilution (PMID 20139179).
IHC-P: 1/50 - 1/100.

WB: 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Is unsuitable for Flow Cyt or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Sequence similarities

Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.

Post-translational
modifications

Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.

Cellular localization

Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.

Target information above from: UniProt accessionP05198 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EIF 2 alpha antibody
  • EIF 2 antibody
  • EIF 2A antibody
  • EIF 2alpha antibody
  • eIF-2-alpha antibody
  • eIF-2A antibody
  • EIF-2alpha antibody
  • EIF2 alpha antibody
  • EIF2 antibody
  • EIF2A antibody
  • EIF2S1 antibody
  • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
  • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
  • Eukaryotic translation initiation factor 2 subunit 1 antibody
  • Eukaryotic translation initiation factor 2 subunit alpha antibody
  • IF2A_HUMAN antibody
see all

Database links

see all

Anti-EIF2S1 (phospho S51) antibody [E90] images:

  Western blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)

Western blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate

Predicted band size : 36 kDa
Observed band size : 36 kDa

  - EIF2S1 (phospho S51) antibody [E90] (ab32157)

- EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

  Dot Blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)

Dot Blot - EIF2S1 (phospho S51) antibody [E90] (ab32157)

Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.

  Immunocytochemistry/ Immunofluorescence - EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunocytochemistry/ Immunofluorescence - EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.

This image is courtesy of an Abreview submitted by Mr mike lindquist

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Breast carcinoma tissue.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

ab32157 showing positive staining in Cervical carcinoma tissue.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

ab32157 showing positive staining in Colonic adenocarcinoma tissue.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EIF2S1 (phospho S51) antibody [E90](ab32157)

ab32157 showing positive staining in Hepatocellular carcinoma tissue.

References for Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

This product has been referenced in:

  • Coope A  et al. Chaperone insufficiency links TLR4 signaling to endoplasmic reticulum stress. J Biol Chem : (2012). WB; Mouse.Read more (PubMed: 22427667) »
  • Cantero-Recasens G  et al. The asthma-associated ORMDL3 gene product regulates endoplasmic reticulum-mediated calcium signaling and cellular stress. Hum Mol Genet 19:111-21 (2010). WB; Human.Read more (PubMed: 19819884) »

See all 8 publications for this product

Publishing research using ab32157? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"