Anti-EIF2S2/EIF2B antibody (ab155649)
Key features and details
- Rabbit polyclonal to EIF2S2/EIF2B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF2S2/EIF2B antibody
See all EIF2S2/EIF2B primary antibodies -
Description
Rabbit polyclonal to EIF2S2/EIF2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human EIF2S2/EIF2B aa 119-331.
Database link: P20042 -
Positive control
- 293T, A431, H1299 and HeLa cell lysates; mouse liver lysate; rat PC12 and Rat2 cell lysates; HeLa cells; Human SG xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
Sequence similarities
Belongs to the eIF-2-beta/eIF-5 family. - Information by UniProt
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Database links
- Entrez Gene: 8894 Human
- Entrez Gene: 67204 Mouse
- Entrez Gene: 296302 Rat
- Omim: 603908 Human
- SwissProt: P20042 Human
- SwissProt: Q99L45 Mouse
- Unigene: 429180 Human
- Unigene: 377134 Mouse
see all -
Alternative names
- DKFZp686L18198 antibody
- eIF 2 beta antibody
- eIF-2-beta antibody
see all
Images
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Paraffin-embedded mouse kidney tissue labeling EIF2S2/EIF2B using ab155649 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution + mouse liver lysate at 50 µg
Predicted band size: 38 kDa
10% SDS PAGE -
All lanes : Anti-EIF2S2/EIF2B antibody (ab155649) at 1/2000 dilution
Lane 1 : PC12 whole cell lysate
Lane 2 : Rat2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
10 % SDS-PAGE -
All lanes : Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
10% SDS PAGE -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SG xenograft tissue, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution. The image in the right panel was costained with Hoechst 33343.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155649 has not yet been referenced specifically in any publications.