Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF2S2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 119-331 of Human EIF2S2 (UniProt ID: P20042).

  • Positive control
    • 293T, A431, H1299 and HeLa cell lysates; mouse liver lysate; rat PC12 and Rat2 cell lysates; HeLa cells; Human SG xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities
    Belongs to the eIF-2-beta/eIF-5 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686L18198 antibody
    • eIF 2 beta antibody
    • eIF-2-beta antibody
    • EIF2 antibody
    • EIF2B antibody
    • EIF2beta antibody
    • EIF2S2 antibody
    • Eukaryotic initiation factor 2 beta antibody
    • eukaryotic initiation factor 2-beta antibody
    • Eukaryotic translation initiation factor 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit 2 antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit beta antibody
    • eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody
    • IF2B_HUMAN antibody
    • MGC8508 antibody
    • PPP1R67 antibody
    • protein phosphatase 1, regulatory subunit 67 antibody
    see all

Images

  • Anti-EIF2S2 antibody (ab155649) at 1/1000 dilution + mouse liver lysate at 50 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • All lanes : Anti-EIF2S2 antibody (ab155649) at 1/2000 dilution

    Lane 1 : PC12 whole cell lysate
    Lane 2 : Rat2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10 % SDS-PAGE
  • All lanes : Anti-EIF2S2 antibody (ab155649) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SG xenograft tissue, labeling EIF2S2 using ab155649 at a 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling EIF2S2 using ab155649 at a 1/500 dilution. The image in the right panel was costained with Hoechst 33343.

References

ab155649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155649.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up