Overview

  • Product nameAnti-EIF2S2 antibody
    See all EIF2S2 primary antibodies
  • Description
    Mouse monoclonal to EIF2S2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MSGDEMIFDP TMSKKKKKKK KPFMLDEEGD TQTEETQPSE TKEVEPEPTE DKDLEADEE DTRKKDASDD LDDLNFFNQK KKKKKTKKIF DIDEAEEGVK D, corresponding to amino acids 1-101 of Human EIF2S2 (NP_003899) with a 26 kDa tag

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctioneIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686L18198 antibody
    • eIF 2 beta antibody
    • eIF-2-beta antibody
    • EIF2 antibody
    • EIF2B antibody
    • EIF2beta antibody
    • EIF2S2 antibody
    • Eukaryotic initiation factor 2 beta antibody
    • eukaryotic initiation factor 2-beta antibody
    • Eukaryotic translation initiation factor 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit 2 antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit beta antibody
    • eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody
    • IF2B_HUMAN antibody
    • MGC8508 antibody
    • PPP1R67 antibody
    • protein phosphatase 1, regulatory subunit 67 antibody
    see all

Anti-EIF2S2 antibody images

  • Anti-EIF2S2 antibody (ab77784) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • Anti-EIF2S2 antibody (ab77784) at 1/500 dilution + EIF2S2 expressed in HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 38 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF2S2 antibody (ab77784)

ab77784 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"